They provide absorbance, fluorescence, glow luminescence, and other read modes for. The Hidex Sense is a compact, state-of-the-art, multi-mode microplate reader which provides a powerful and flexible platform for performing any kind of assay without limitations. , Sunnyvale, CA, USA). Related Instruments Agilent BioTek BioStack microplate stacker Automate routine microplate washing or dispensing processes with the compact BioStack microplate stacker. HEK-Blue-hTLR4 and HEK-Blue-hTLR2 cells were incubated with. 2. 100 fmol 1536-well (8 µL) 0. The data were analyzed using Multi-Mode Analysis software (Molecular Devices, Corp. After 2 hours, each well was transferred to a 96-well flat-bottom plate and the absorbance at 490 nm was measured on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA). 100 fmol 384-well (75 µL) 0. LVF Monochromator™ : Excitation and Emission. 8 Pages. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. The plates were loaded into a FilterMax TM F5 Multi-Mode Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA), and the fluorescence intensity was measured at 37 °C with Ex/Em = 440/484 nm and 15 s shaking between reads to facilitate aggregation. SpectraMax Quant dsDNA Assay Kits. 561. Technical specifications Compatible with these Molecular Devices systems Contact Us Phone: +1-800-635-5577 Email: [email protected]. Cells were seeded in 24-well plates and cultured. After incubation for 2 h at 37 °C, the mixture was centrifuged at 2200 rpm for 10 min to obtain the supernatant. $3,000 USD. BMG POLARstar Microplate Reader, 115-230V, BMG Labtech (CAT#: STEM-SPO-0061-YJL) Perkin Elmer Victor3 1420 Multilabel Counter, 110-240V, 3 Amps, PerkinElmer (CAT#: STEM-SPO. Description: The FilterMax™ F3 features absorbance (visible),. Laser-aided direct metal deposition of metals and alloys. HERM LB 500 with NaI detector Radio HPLC Detector. Luminescence intensity was measured with a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). Important things to consider when purchasing a multimode microplate reader. It can be upgraded into a powerful multi-mode reader with up to seven detection modes. 3 Pages. shopping cart 0 items $0. Supernatant (25 μL), or standard, was added and mixed. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. After 30 min, the reaction was stopped by adding 1 N HCl and absorbance at a wavelength of 490 nm was measured using the FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Corp. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell. Plate Formats:. , Sunnyvale, CA, USA) and normalized to the. Add to my manuals. Page: 1/4 NADPH turnover was measured on FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Francisco, USA) at 340 nm over 30 min at 37 °C. Conquer Scientific provides laboratories around the world high quality, low-cost pre-owned laboratory instruments. Add to cart. The amount of colorimetric product was continuously monitored for 120 min. In addition, the FilterMax F5 Reader is capable of measuring. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. The increase in NADH, an end product in this reaction, was determined by means of its light absorbance at 355 nm using a fluorometric microplate reader (FilterMax F5 Multi-Mode Microplate Reader; Molecular Devices,. F3/F5 Multi-Mode Microplate Readers. 00. Fluorescence intensity was determined using a FilterMax F5 multi-mode microplate reader at an excitation wavelength of 485 and an emission wavelength of 530nm. SpectraMax Gemini XPS/EM Microplate Readers. We have 2 Molecular Devices FilterMax F5 manuals available for free PDF download:. shopping cart 0 items $0. This can increase throughput and efficiency, thus saving time and space in the lab. Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535. Page 25: FeaturesThe Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seIMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. Click “Eject Plate Carrier. 3 Pages. LDH cytotoxicity assaySome of new FilterMax F5 Multi-Mode Microplate readers are shipped with ARP USB to serial port adaptor (Manufacturer No: 572213, ARP No: 465338, 1 X USB, 1 X serial DB9/m, blue). 4 (Molecular Devices, Sunnyvale, CA, USA) at room temperature (fluorimeter settings used were as follows: measurement type, kinetic, 60 cycle, 60-s interval; excitation wavelength, 360 nm;. MetaMorph NX. cruzi incubated for 1 h with sertraline at 2 μM. F3/F5 Multi-Mode Microplate Readers. The absorbance of samples at 405 nm (A 405) was determined at room temperature at 20 and/or 60 min after addition of substrate using a FilterMax F5 Multi-Mode Microplate reader (Molecular Devices, San Jose, CA). GenePix 4300/4400 Microarray Scanner. SpectraMax Glo Steady-Luc Reporter Assay Kit. Selecting your dsDNA assay kits The family of SpectraMax Quant dsDNA Assay Kits consist of three kits each designed for a specific range of DNA concentrations and optimized for use with florescenceF3/F5 Multi-Mode Microplate Readers. Manufacturer: Molecular Devices. Other technologies such as imaging. SpectraMax Quant dsDNA Assay Kits. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. FilterMax F5 Multi-Mode Microplate Reader. . SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. FilterMax Multi-Mode Microplate Readers Functionality FilterMax F3 FilterMax F5 Absorbance (ABS ) Visible 4 4 Absorbance (ABS) UV 4 Fluorescence intensity (FI) top read 4 4 Fluorescence intensity (FI) bottom read 4 Luminescence (LUM) glow 4 4 Fluorescence polarization (FP) 4 Time-Resolved fluorescence (TRF) 4 Shaking 4 4 Temperature. org) with optimized instrument settings b. A dual-color pyrometer with a selected wavelength away from that of the laser radiation has been used to monitor the melt pool temperature. #F5) Methods Standard curves were generated following the assay protocols provided with each kit. 5 min for 30 min at 340 nm absorbance using a microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, FilterMax F5 Multi-Mode Microplate Reader) and Multi-Mode Analysis Software (Molecular Devices, RRID:SCR_014789), version 3. Luminescence (glow) Fluorescence polarization. Biofilm quantification and scanning electron microscopy (SEM) analysis. 7°C and the bottom to 36. The following document is intended to demonstrate setup of this instrument. Click Run. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among. A Vision for the Future With TTP LabTech. Topic. With optimized reagents, option to operate the. 7/4/2018 Great Multi-Mode Microplate Reader Chirag Patel Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. & FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe OD 405 was measured in a FilterMax™ F5 Multi-Mode Microplate Reader. MS-3 Laser Bar Code Reader. – ™FilterMax F5 Multi-Mode Microplate Reader (cat. In the list below the slide number 21, select the positions where the emission filters are installedF3/F5 Multi-Mode Microplate Readers. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® Europium• FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. Lonza Luminometers Instrument MycoAlert™ Assay MycoAlert™ PLUS Assay Other Bioassays* Notes Lonza Lucetta™ Luminometer + + Cuvette Tailor -made MycoAlert™ Mode Luminometers from Other SuppliersTriplicate reactions were measured every 2. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). KG (CAT#: STEM-LE-1659-Y)The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seAfter 2 hours, each well was transferred to a 96-well flat-bottom plate and the absorbance at 490 nm was measured on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA). The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. Then, 50 µL of a stop solution were applied to terminate the reaction. After incubation, each microplate was analyzed for absorbance reading. M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. Supernatant (25 μL), or standard, was added and mixed. The OD value of each tube (230 μL) was measured in a 96-well microplate at 405 nm using a. 00. , Sunnyvale, CA, USA) for 30 min and 1 h after dye addition. Neurotransmitter Transporter Uptake Assay Kit. Protease activities were calculated from the slope of the linear portion of the normalized fluorescence vs. 2 mm) was measured by a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) every 15 min over 16 h incubation time. After 30 min, absorbance of the lysates at 405 nm was measured on a microplate reader (FilterMax F5 Multi-Mode microplate reader). Datapaq Express (Vial Rack Bottom Label 2D Reader) XTR Rack Pro 96 (Vial Rack Bottom Label 2D Reader) BIO-LAYER INTERFEROMETRY MICROPLATE READING. Download Table of Contents. This document is. Imaging by immunofluorescence. A 1:1 binding ratio Hill model was used to fit the binding interaction data. VisionMate 96 (Vial Rack Bottom Label 2D Reader) Ziath. After 24 h, the. BMG LABTECH - CLARIOstar Plus . Page 1 Hybrid Multi-Mode Microplate Reader Synergy ™ Operator’s Manual. Briefly, 2 h after MTS incubation at 37 °C, absorbance at 490 nm was recorded using a microplate reader (FilterMax F5, Multi-Mode Microplate Reader, Molecular Devices, San Jose, MO, USA). The fluorescence was determined using a fluorimetric microplate reader (FilterMax F5 Multi-Mode Microplate Reader) at 540 and 595 nm for excitation and emission, respectively. , GFP expression yields from High Five cells infected with the two viruses were estimated using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA). LabX is a multi-vendor marketplace that connects you with the life science tools and chemistry you need to run your lab. After 30 min, absorbance of the lysates at 405 nm was measured using a microplate reader (FilterMax F5 Multi-Mode microplate reader). A Vision for the Future With TTP LabTech. *** (p < 0. SoftMax Pro GxP Compliance Software. A powerful and reliable microplate reader is what you need! Get access to readers which will ensure an optimal readout. The readings of samples incubated with different carbon sources were subtracted from the average of the control readings. Microplate readers, multi-mode, FilterMax™ F3 and F5 Supplier: Molecular Devices Description: The FilterMax™ series of multi-mode microplate readers can be operated as standalone instruments or can be integrated seamlessly with laboratory autom. Based on a rugged, simple design, the VMax Absorbance Microplate Reader offers a mean time between failure (MTBF) of 20,000 hours with 97. 40266 Email: drugdiscoverytech@lifetech. URL of this page: HTML Link: Bookmark this page. An anti-human HER2/neu human IgG3/κ (Huang and Morrison, 2006) was used as isotype (negative) control. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. FilterMax F5 Multi-Mode Microplate Reader. Mettler Jr. shopping cart 0 items $0. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Add to cart. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. The following document is. : 28 Sept 12 Page 2 of 20 Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The present study found competitive inhibition of hMAO-A by ILG along with a similar mode of action against hMAO-B as in rMAO-B. An MTT assay was performed with 5 mM BHB in the presence or absence of LPS (1 µg/mL) for 24 h. FlexStation 3 Multi-Mode Microplate Reader. ** Some compatible filter sets are available for purchase from Life. Homogeneous Time-Resolved Fluorescence (HTRF) . SpectraMax iD5. 020 fmol 0. Optical System: High energy xenon flash lamp. 4. Fluorescence kinetics were monitored from the top in a FilterMax F5 Multi-Mode Microplate Reader and the Multi-Mode Analysis Software 3. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Cells were co-transfected with 2 μg total plasmid DNA (1760 ng empty vector or proviral plasmid DNA. Turn on the power to the FilterMax F5. C1–F5 Filter Set: EX 360/40 nm EM 460/40 nm Optics Position: Gain: Auto Scale to High Wells Scale Well: C1 Scale Value: 30000 Delay after Plate Movement: 100 msec Synergy. Page 1 SpectraMax® M3 SpectraMax® M4 SpectraMax® M5 SpectraMax® M5e Multi-Mode Microplate Readers User Guide 0112-0115 G October 2018. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solu. 005 OD. d. 8 Pages. 3. Method Fluorescence intensity Read Type Endpoint / Kinetic Optics Type Filters Step Label "Linearity Read" Read well C1-F5 Filter Set. 4 Pages. The planktonic growth inhibition (%) was calculated according to the following equation:Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. Transfluor Technology. The USB adaptor comes with 1 X CD that contains its USB driver. 3. HTRF-compatible SpectraMax Multi-Mode Readers. Supplier: Molecular Devices. Background light emission was measured using the luminescence setting on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Techniques). 09 CAD. Fold changes were calculated as the area under the curve of stimulated HKLs relative to unstimulated HKLs (RPMI without zymosan). SP Bel-Art Petri Dish Plate Reader, Bel-Art Products, a part of SP . They provide absorbance, fluorescence, glow. 1% (v/v) formic acid, was adopted for lipid separation: 0–2 min. Synergy HTX and Synergy LX. 400. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. MetaFluor Software Brochure. Add. Create an experiment based on the protocol. microplate reader. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. & FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. time plots at 30, 60, and 90 min using MATLAB® R2017b (9. Samples were considered positive if the average sample absorbance was greater than twice the mean absorbance of healthy. The transmittance at 450 nm was determined by FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA). 3 Pages. F3/F5 Multi-Mode Microplate Readers. 2. 012 fmol 0. 2 Pages. BMG Labtech - FLUOSTAR OMEGA. 4 million by 2027; it is expected to grow at a CAGR of 3. 5. Synergy HT laboratory equipment pdf manual download. please click here. Filters: Excitation and emission slides for 4 filters each. The amount of the colorimetric product was monitored continuously for 120 min. Page 154: Ordering Parts And Accessories. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader $7,900. c. FilterMax F3 & F5 Multi-Mode Microplate Readers. Plates were read at absorbance 405 nm using a FilterMax F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). The transmittance at 450 nm was determined by FilterMax F5 Multi-Mode Microplate Reader (Molecular devices, CA, USA). ABS Test results. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. 5. SpectraMax iD5. 4 Pages. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of. Depending on the model, Cytation 5 detection modes include fluorescence intensity (FI), fluorescence polarization (FP), time-resolved fluorescence (TRF), luminescence, UV-visible absorbance, imaging, and alpha. FilterMax F3 & F5 Multi-Mode Microplate Readers: SpectraMax i3x Multi-Mode Microplate Reader: SpectraMax Paradigm Multi-Mode Microplate Detection System: Citations: Figures: Catalog Number: Inquire: FilterMax F3 / FilterMax F5: i3x: Inquire: Price: Description: The SpectraMax® i3 Multi-Mode Detection Platform comes with standard. 7. Molecular Devices 1311 Orleans Drive Sunnyvale, California 94089 Telephone: +1-408. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. 4 Pages. 561. FilterMax™ F3 and F5 Multimode Microplate Readers, Molecular Devices. FilterMax F3/F5 Multi- Mode Microplate Readers · FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: of 6 /6. Multi-Mode Readers. Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader + + Molecular Devices FlexStation. Instrument Configuration: Assay Technology Adapta® & FlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer Key Features • Five-mode reader with wide range of applications • Flexible liquid transfer enables more assay conditions • User-defined pipetting simplifies assay optimization • Instrument and software validation Adapting to biochemical- and cell-based application requirements while. SpectraMax Quant dsDNA Assay Kits. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader w/ One Year Warranty (Svc Contract Available)Find used microplate readers on Machinio to perform a wide range of assays including ELISAs, cell viability assays, nucleic acid quantification, and more. FilterMax F3 & F5 Microplate Readers. Cytokines Assay. Set up plate with cells, reagent and test compounds. FilterMax F3/F5 Multi- Mode Microplate Readers · FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: of 6 /6. Time-resolved Fluorescence. 6. #0200-7030) Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. 713579). The next day, precultures were diluted to an optical density of 0. Published Dec 16, 2020 | Updated Dec 16, 2020. MetaXpress. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Time-resolved Fluorescence. ** Some compatible filter sets are available for. 7°C (gradient heating design). Absorbance was measured at 485 nm with FilterMax F5 Multi-Mode microplate readers and the percentage of viable cells was calculated in relation to cells cultured in pure medium. Open SoftMax® Pro software. BMG Labtech - THERMOstar. 8 Pages. The amount of colorimetric product was continuously monitored for 120 min. Caspase-3 Activity. All ELISA kits were read using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices; San Jose, CA, USA), according to the kit guidelines. 00. SpectraMax Mini Multi-Mode Microplate Reader. ; Page 2 For research use only. Molecular Devices, LLC Reading Modes: Endpoint, Kinetic, Linear Scan, Area Scan; Optical System: LEDs, Deuterium Lamp; Wavelength Range: 230 to 750 nm;. Molecular Devices Multi-Mode Microplate Reader FilterMax F5 $7,995. Renilla/Firefly luciferase activities were measured twice. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe OD 600 of the culture in the plate was measured by a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) every 20 min over 20 h. ABS Test results. Caspase-3 activity was used as a marker of cell apoptosis and was assessed according to. Samples (180 μl) were stained with 20 μl of Nile red (2. 100 fmol 384-well (75 µL) 0. 425 or. SpectraMax Paradigm Multi-Mode Microplate Reader. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. The results were calculated using a malondialdehyde (MDA) curve and expressed as nmol MDA/mg protein. The medium was then removed and the cells washed once with 200 μl of ice-cold phosphate buffered saline (PBS). Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Yes, the M5 does have a limited slip. At day 4 P. FilterMax F5 Multi-Mode Microplate Reader. The Labsystems Nepheloskan Ascent Microplate Reader is designed to run with 96-well microplates, for use on applications like immunoprecipitation reactions as well as solubility tests of pharmaceutical components. The amount of each HTRA1 protein was. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. FilterMax F5 Multi–Mode microplate reader from Molecular Devices Multi–Mode Analysis software. Luminoskan luminometric microplate reader. 3 Pages. . : F5: Accuracy: \ Detection Limit (384-Well): 0. 3 Pages. To read the absorbance, a spectrophotometer (Filter Max F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA 95134, USA) with a wavelength of 595 nm was used. Fluorescence of the organic phase was measured at 515 nm of excitation and 553-nm emission using a fluorimeter (FilterMax F5-Multi-Mode Microplate Reader, Molecular Devices, CA, USA). Select preinstalled protocol on the GloMax® Instrument and pre-warm instrument. Learn how to set up protocols, customize reports, export results, and more with this PDF document. The user guide also covers the software features, the maintenance procedures, and the technical specifications of the readers. Drug repurposing has been an interesting and cost-effective approach, especially for neglected diseases, such as Chagas disease. FilterMax F5 and F3 Multi‐Mode Microplate ReadersUnpackingand Setup Guide 5016209 B 3 Setting Up the Instrument Setting up theinstrument includes selecting asuitablework area, installing excitation and emission filter slides, connecting thehost computer, and installing thesoftwarethat controls allactions performed by the instrument. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Preconfigured SoftMax® Pro Software protocols (available at softmaxpro. By category. FilterMax® F3 & F5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell viability, ELISA, Fluorescence,. Coculture experiment. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. The hemolysis was calculated with the following formula:Luminescence from the converted ATP was then measured using a FilterMax F5 Multi-mode microplate reader (Molecular Devices). 0. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers can measure absorbance, fluorescence and luminescence. SpectraMax Glo Steady-Luc Reporter Assay Kit. : 28 Sept 12 Page 2 of 20 Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The OD 600 was plotted against time using Graphpad Prism 8. Absorbance was detected from the bottom using the FilterMax F5 multimode microplate reader (Molecular Devices, Sunnyvale, CA) at excitation/emission wavelengths of 485 nm/535 nm. This assay measures cellular metabolic activity as an indicator of cell viability and proliferation. ” e. 2. , Ltd. Upgradeable modules are also available including western blot, cell imaging, and fast. 5% (v/v) to avoid toxicity. Bioprocess Equipment and Technology. Instrument Configuration: Assay Technology Adapta® & The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. 25. book Page 1 Tuesday, September 7, 2010 6:14 PM. The fluorescent signals were measured at 10 minutes and one hour after the Stoplight Red substrateFilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) 12. SpectraMax Glo Steady-Luc Reporter Assay Kit. , a long-time leader in bioanalytical systems for microplate analysis, today announced the launch of two new multi-mode detection platforms that expand its portfolio of microplate readers, enabling the company to meet all combinations of customer needs now and into the future. Then 100 μL of 2 mol L −1 sulphuric acid was added to stop the enzymatic reaction and the absorbance values were read at 450 nm on a FilterMax F5 Multi-Mode Microplate Reader. Detection cartridge modularity allows for easy addition of new read modes within minutes without. 00. These assays span over a wide wavelength range, generally from 220-900 nm in different detection modes (typically absorbance, fluorescence and luminescence). Microplate formats can vary from 6-1536 wells, providing the ability to read many samples in a short time. 4. The quantity of colorimetric product was continuously monitored for 120 min. Synergy™ H1 is a flexible monochromator-based multi-mode microplate reader. 3 Pages. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. The ucs load command creates a backup of. Sign In Upload. Selecting your dsDNA assay kits The family of SpectraMax Quant dsDNA Assay Kits consist of three kits each designed for a specific range of DNA concentrations and optimized for use with florescenceThe SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. A standard curve was created by diluting the ATP standards to a range between 2 × 10 −3 and 20 μM ATP. 3 Pages. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Discover Precision and Reliability with Our Featured General Laboratory Instruments - Thermo Forma 3110 Water Jacketed CO2 Incubator, Thermo ULT185-5-V -80 Freezer, Filtermax F5 Multimode Microplate Reader, Spectramax M5e, Thermo Napco CO2 Incubator, and XS 64 Balance. Molecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life Technologies’ GeneBLAzer® assays. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The ELISA test was performed using the supernatants from the morphological test. Instrument Configuration: Assay Technology Adapta® & Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET only) (TR-FRET only) HTRF Certification LanthaScreen Certification Key. 7. Unsuccessful migration. Thereafter, the dye liquid was removed, the formazan was solubilized with dimethyl sulfoxide (DMSO) under continuous agitation for 15 min, and the absorbance of each well was read by the means of a microplate reader (FilterMax F5 Multimode Microplate Reader; Molecular Devices) at a wavelength of 570 µm. The standard curve presented a linear phase from 1. 2 Pages. 4 Pages. The FilterMax F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), time-resolved fluorescence (TRF), for 6- to 384-well plates. Match case Limit results 1 per page. Compact designs make these instruments. All experiments were performed in triplicate. . After further centrifugation in the same fashion, the supernatant was subjected to subsequent enzymatic reactions. There is no programming of protocols or wet chemistry involved –just select the mode(s) you want to test, insert the plate and go. Renilla luciferase activity was normalized to the background Firefly luciferase activity produced from the same psiCHECK-2 vector for each condition. Synergy™ H1 Hybrid Multi-Mode Microplate Reader, Wavelength Range: 200 nm to 999 nm, BioTek Instruments (CAT#:. Each measurement was repeated at least 3 times, and IC 50 values were calculated by non-linear regression in GraphPad Prism 9. Share. 425 or. The SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. Once the FilterMax F5 has completely powered on, double-click on the Multi- Mode Analysis Software. Consult Molecular Devices's entire F3/F5 Multi-Mode Microplate Readers catalogue on MedicalExpo. Enzymatic activity was calculated after subtracting absorbance decay obtained from buffer without cell lysates by using NADPH extinction coefficient of 6220/M/cm and by normalizing to total protein. 1 MB Table of Contents. Not for use in diagnostic procedures. F3/F5 Multi-Mode Microplate ReadersCell pellets were lysed and HTLV-1 LTR-transactivation was measured via luciferase assay according to the manufacturer’s protocol (Dual-Luciferase ® Reporter Assay System, Promega Corporation, Madison, WI; Filtermax F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA) . SpectraMax Paradigm Multi-Mode Microplate Detection System from Molecular Devices, LLC. Gently pull the plastic tab protruding from the microplate chamber door. 3 Pages. State-of-the-art Multi-Mode Microplate Reader. The FlexStation 3 Benchtop Multi-Mode Microplate Reader can measure absorbance, fluorescence intensity, fluorescence polarization, luminescence and time-resolved fluorescence. Measurement of Caspase 3/7 Activity. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumAfter 30 min, the absorbance of the lysates was measured at 405 nm using a FilterMax F5 Multi-Mode microplate reader. You can also contact Technical Support by email. The Evans blue dye was extracted from the ears with 1 ml of formamide overnight at 55°C and measured spectrophotometrically at 620 nm (FilterMax TM F5 Multi-Mode Microplate Reader). 3 Pages. SpectraMax® M5/M5e Microplate Reader & FlexStation® 3 Benchtop Multi-Mode Microplate ReaderSpectraMax M-series Microplate readers. The values of luminescence are directly proportional to the number of viable cells. Caspase 3/7 activity was performed as previously described [8,9,10,20]. We specialize in GC, IC, GC/MS, HPLC, LC/MS, Spectrophotometer, and PCR Systems. Open SoftMax® Pro software. Guiberteau MD, in Essentials of Nuclear Medicine Imaging (Sixth Edition), 2012 Photon Transducers. Figure 2: Images of colonies of commonly used cell types raised in semi-solid culture in CloneMedia.